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Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain <t>DNA</t> from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).
Orange Dna Loading Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/orange dna loading dye/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
orange dna loading dye - by Bioz Stars, 2026-03
95/100 stars
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orange g loading dye  (LI-COR)
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LI-COR orange g loading dye
Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain <t>DNA</t> from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).
Orange G Loading Dye, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/orange g loading dye/product/LI-COR
Average 98 stars, based on 1 article reviews
orange g loading dye - by Bioz Stars, 2026-03
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orange dna loading dye 6x  (Thermo Fisher)
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Thermo Fisher orange dna loading dye 6x
Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain <t>DNA</t> from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).
Orange Dna Loading Dye 6x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/orange dna loading dye 6x/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
orange dna loading dye 6x - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
6x orange dna loading dye  (Thermo Fisher)
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Thermo Fisher 6x orange dna loading dye
Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain <t>DNA</t> from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).
6x Orange Dna Loading Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6x orange dna loading dye/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
6x orange dna loading dye - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
6x orange dna loading dye 12  (Thermo Fisher)
95
Thermo Fisher 6x orange dna loading dye 12
Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain <t>DNA</t> from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).
6x Orange Dna Loading Dye 12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6x orange dna loading dye 12/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
6x orange dna loading dye 12 - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier

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Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain DNA from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).

Journal: Frontiers in Plant Science

Article Title: Large scale deletion and rebalancing within the k1C kafirin family in sorghum

doi: 10.3389/fpls.2025.1686027

Figure Lengend Snippet: Polymerase chain reaction detection of breakpoint region in (A) 19Q4–77 wx and RTx430 and (B) 19Q4–94 Wx and RTx430 and Integrative Genome Viewer screenshot of contiguous assemblies of 19Q4–77 wx , 19Q4–94 Wx , and RTx430 reads at and around the ~400-kb deletion in k1C family (C) . Top row depicts amplification of unedited genome using primer set in deleted region (716 bp), and bottom row depicts amplification of junction fragment from edited genome using primer set that spans deletion (780 bp). First and last lanes of each row contain a 1-kb ladder. Lanes 2–14 for top and bottom rows contain DNA from 19Q4–77 wx (A) and 19Q4–94 Wx (B) plants, with lanes 17–19 containing DNA from unedited RTx430 plants (both).

Article Snippet: PCR products were combined with Orange DNA Loading Dye (Thermo Fisher; Catalog no. R0631: Massachusetts, United States) and loaded onto 1%–2% agarose gels stained with ethidium bromide.

Techniques: Polymerase Chain Reaction, Amplification